Exciting Discoveries in Gel Electrophoresis!

What type of gel should be run for DNA fragments below 100bp?

1) Agarose gel
2) Polyacrylamide gel
3) Both Agarose gel and Polyacrylamide gel
4) Neither Agarose gel nor Polyacrylamide gel

Answer:

Agarose gel should be used to run DNA fragments below 100bp.

When running DNA fragments below 100bp, it is recommended to use agarose gel. Agarose gel is a common choice for gel electrophoresis as it allows for the separation of DNA fragments according to size. The gel is poured into a casting tray and solidified before loading the DNA samples.

The DNA samples are then subjected to an electric current which causes the DNA fragments to move through the gel. The smaller fragments move faster and farther from the well, while larger fragments move more slowly and stay closer to the well. This separation based on size helps in analyzing and identifying DNA fragments of interest.

Agarose gel is an ideal choice for separating DNA fragments below 100bp due to its ability to provide clear resolution between small DNA fragments. By using agarose gel, researchers can accurately determine the size range and quantity of DNA fragments present in their samples.

In conclusion, agarose gel is the preferred choice for running DNA fragments below 100bp during gel electrophoresis, as it enables efficient separation and analysis of DNA samples based on their size. This technique has revolutionized the field of molecular biology and continues to pave the way for exciting discoveries in genetics and genomics.